Very little was released publicly regarding the plot of the remake and the extent to which it would be faithful to the original. It was expected that the central focus would be on three teenagers in a family of vampires traveling Route 66 in the late 1980s.
The plot line focuses on the Lassader family, headed by Joe and Carol, whose daughter Selena searches out Derek McGregor and his team to hunt and kill a vampire colony that is holding the family hostage by drinking the blood of their livestock, the source of their income. Accepting the offer, McGregor and his small team begin a bloody, terrifying journey to track and destroy the evil vampire brood, with the help of vampire specialist Xavier Burns.
Count Murnau, a wealthy eccentric from the Carpathian region wishes to buy a property in Hannover, Germany. The real estate agency sends Mr. Dohart, his best sales representative to close the deal, but Dohart never gets back. The real state agency sends the young and newlywed Hutter to take care of this matter, and he departs alone to Transylvania leaving his wife Ellen waiting for his return.
Klaha and Cecil are set to get married. However, their wedding must be postponed because Klaha is summoned from England to Romania on business. Klaha goes to Transylvania, and arrives at the Earl of Dracula's castle, where he is staying. The Earl seems welcoming, but Klaha has strange experiences while staying there. On his arrival, he notices a painting of the Earl's deceased wife, who looks very much like Cecil. 2 nights later, the Earl agrees to buy a house in England. That same night, after having a nightmare, Klaha wakes up to see a coffin leaving the grounds. While looking for the Earl, Klaha sees the painting again, but this time, the word "Cecil" is written under it. Too late does Klaha realise that the Earl is a vampire. He immediately leaves to try to catch up with him.
A feminist blogger and reporter for the school newspaper, tries to stop Countess Elizabeth Báthory, who poses as an abstinence counselor in a high school, from killing the school's virgins to stay young and beautiful.
Gretchen (Sean Serino), a young woman, becomes desperate to contact her brother after his passing. She discovers an amulet that allows her to control the dead—which she uses to try and contact him. The amulet also allows results in the calling forth of a small army of zombies, comedically called "Post-Mortem Americans" in the film. This amulet is a sought after relic, pursued by two feuding vampires who have been at odds for millennia: Vellich (Andrew Divoff) and Sebed (Tom Savini). After encountering the vampires, Gretchen and her friends are joined by the vampire-hunter McCallister (Jason Carter), who had originally stolen the amulet from Vellich. McCallister helps Gretchen and her friends combat the villains, and the film ends with a final showdown between the protagonists and the vampires. The film was called a "vampire-zombie hybrid movie" by the Columbus Dispatch.
Barrow, Alaska is preparing for its annual "30 Days of Night", a period during the winter when there is a month-long polar night. As the town gets ready, a stranger rows ashore from a large ship. Once ashore, he sabotages the town's communications and transport, destroying all means of communication with, and travel to, the outside world. Barrow's sheriff, Eben Oleson, investigates. Eben learns that his ex-wife, Stella Oleson, missed the last plane and must stay the 30 days. Although they try to avoid one another, when Eben confronts the stranger in the town diner, Stella helps to subdue him and take him to the station house.
A year after the Alaskan town of Barrow's population was decimated by vampires during its annual month-long polar night, Stella Oleson (Kiele Sanchez) travels the world trying to convince others that vampires exist. She is fully aware of the risk to the life that her work could bring, but does not care due to her grief over the death of her husband Eben.
A young, hardworking Iranian man named Arash lives with and takes care of his heroin addicted father, Hossein. They are harassed by a cruel, drug-dealer pimp named Saeed, who seizes the young man’s prized car in exchange for money the father owes him. In a crime of opportunity, Arash steals a pair of diamond earrings from the wealthy young woman he works for. The pimp comes across a strange, young woman in a chador at night, and back at his apartment she grows long fangs and goes for his neck. As she leaves she passes by the young man, who has come to offer the earrings for his car. He finds the drug dealer dead, taking back his car keys, along with the drugs and cash. The young man dresses up as Dracula, and goes to a night club where he is seduced into taking one of the “X” pills he is selling by a young woman he knows. Under the influence, he is rejected by her, and ends up lost at night on the street.
In a posh London gallery, Carolyn Dalgleish (Carolyn Backhouse) prepares a showing of the latest works by John Bolton; disturbing portraits of beautiful, vicious vampiric women he encountered whilst pot holing. The Interviewer (Marcus Brigstocke) collects information on Bolton, who seems to perplex those who work with him and collect his art (like radio personality Jonathan Ross (playing himself)).
A Miami businessman, John Stone, receives a parcel from England containing two old bottles of Slivovitz brandy from his recently deceased ancestor, and after drinking both bottles, becomes a vampire. Stone uses his newfound vampire powers to keep his wife, Helena, in a trance as he travels to England to kill the descendents of Abraham Van Helsing that murdered Count Dracula. Meanwhile, Helsing's distant relative, Howard Helsing, pursues Stone with the intent to put the reborn vampire to rest for good.
In 1818, Abraham Lincoln (Benjamin Walker) lives in Indiana with his parents, Nancy (Robin McLeavy) and Thomas (Joseph Mawle), who works at a plantation owned by Jack Barts (Marton Csokas). There, Lincoln intervenes when he sees his friend, a young African American boy, William Johnson (Anthony Mackie) being beaten by a slaver. Because of his son's actions, Thomas is fired. That night, Lincoln sees Barts break into his house and attack his mother. Nancy falls ill the following day, and dies shortly afterwards. Thomas tells Lincoln that Barts poisoned her, but asks that he promise not try to avenge her death.